The development of a novel copper-loaded mesoporous silica nanoparticle as a peroxidase mimetic for colorimetric biosensing and its application in H2O2 and GSH assay.

In recent years, the development of nanomaterials-based peroxidase mimics as enzyme sensors has been attracting considerable interest due to their outstanding features, including potent stability, and cost-effectiveness toward natural enzymes. In this work, mesoporous silica nanoparticles functionalized by copper (Cu-MSN) were prepared as a new artificial enzyme for the first time through the sol-gel procedure. A comprehensive investigation of the catalytic activity of Cu-MSN was done through the oxidation of chromogenic peroxidase substrates, 3,3',5,5'-tetramethylbenzidine (TMB), and (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), in the presence of H2O2. The results indicate that the peroxidase-like activity of the as-prepared sample is significantly higher than other nanoparticles. Additionally, for the study, a facile and rapid sensing method based on the enzyme-like activity of Cu-MSN to detect H2O2 and glutathione (GSH) was developed to examine the potency of the proposed biosensor. Preliminary analysis revealed that the limit of detection (LOD) of H2O2 and GSH is 0.2 and 0.0126 µM, in the range of 0.9-100 and 0.042-1 µM, respectively. These findings support the claims for the efficiency of the sensor in detection fields. Also, human serum was utilized as the real sample to obtain additional evidence.

A novel colorimetric sensor for naked-eye detection of cysteine and Hg2+ based on "on-off" strategy using Co/Zn-grafted mesoporous silica nanoparticles.

In an attempt to explore the significance of inorganic mimetic enzymes as sensors, this study introduces a naked-eye analytical sensing platform for the detection of L-cysteine (cys), mercury ions (Hg2+) based on (turn off/turn-on) catalytic activity of zinc and cobalt grafted mesoporous silica nanoparticles (MSNs). To this end, Zn-MSN and Co/Zn-MSN catalysts were synthesized and characterized using XRD, FT-IR, FESEM, TEM, and nitrogen adsorption-desorption methods. Then, using the intrinsic peroxidase-like activity of as-synthesized samples, the oxidation reactions of the chromogenic substrate (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)) was designed using H2O2, which produced green colored cation radical of ABTS. Considering the high peroxidase-like activity of Co/Zn-MSN in comparison to Zn-MSN, it was employed to detect cys and then Hg2+. The results indicated that the strong interaction between cys and Co/Zn-MSN was proved by a limit of detection (LOD) down to 0.24 nM and the linear relationship from 0.8-50 nM (turn off). Given the fact that Hg2+ has a high-affinity tendency to combine with cys, we were suggested a novel colorimetric path for sensing of Hg2+ in the presence of cys (turn on). Based on this method, LOD was found 0.17 nM with the linear range of 0.57-50 nM. Taken together, results showed that the as-prepared catalysts are superior to other nanoparticles as a sensor to measure the target molecules in biological monitoring and clinical diagnostics.

Tailoring cysteine detection in colorimetric techniques using Co/Fe-functionalized mesoporous silica nanoparticles.

Over the past decade, there has been a dramatic increase in the number of studies focused on sensors for cysteine (Cys) as a crucial factor in physiological function and disease diagnosis. Among those sensors, nanomaterial-based peroxidase mimetics have received particular attention from researchers. This study introduces a new series of mesoporous silica nanoparticles (MSNs) incorporated with iron and cobalt (Co/Fe-MSN) with a molar ratio of Si/Fe = 10 and cobalt species at 1, 3, and 5 wt% that have great potential in the sensing application. These nanomaterial characterization was investigated by FTIR spectroscopy, SEM, TEM, XRD, and nitrogen adsorption-desorption. The peroxidase activity of these nanomaterials was studied through kinetic analysis. The findings revealed that Co/Fe-MSN (1%) showed higher peroxidatic activity than the others towards the common chromogenic substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt. Based on the enzymatic activity of Co/Fe-MSN (1%), a colorimetric sensing platform was designed to detect H2O2 and Cys. The limit of detection (LOD) for H2O2 and Cys was determined to be 1.1 µM and 0.89 nM, respectively. The results indicated that the proposed enzyme mimic exhibited excellent potential as a sensor in medical diagnostics and biological systems.